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The effects of the iron ions on the light-induced mitochondrial F₁-ATPase in Lentinus edodes was studied. This enzyme activity was stimulated by each of the ferric, ferrous and magnesium ion. Especially, the activity of the enzyme by 5.0 mM ferric ion increased up to 107% in comparison with control group(100%). In the presence of magnesium ion, each of ferric and ferrous ion increased the activity of the enzyme, particularly, coexistence of 0.1 mM magnesium and 5.0 mM ferric ion increased the activity up to 270% with magnesium ion dependence. The activity of the enzyme was stimulated up to 268% by 5.0 mM ferric ion in the presence of 0.1 mM magnesium and 0.1 mM ferrous ion. Therefore, the coexistence of ferrous ion did not affect the activity. From the above, we propose that light-induced mitochondrial F₁-ATPase in Lentinus edodes is a Mg^(2+) Fe^(3+) F₁-ATPase. The optimal pH and temperature for the enzyme were 7.5 and 66℃ respectively.
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